Test Bank Chapter 20 DNA Technology and Genetic Engineering

Human Biology: Concepts and Current Issues, 8e, (Johnson)

Chapter 20 DNA Technology and Genetic Engineering

20.1 Multiple-Choice Questions

1) Manipulation of the genetic makeup of cells or whole organisms is known as

A) genetic analysis.

B) DNA splicing.

C) molecular biology.

D) genetic engineering.

E) genomic splicing.

Topic: Sec. 20.0

Bloom's: Remembering/Understanding

LO: 20.3a

GLO: G1

2) The rapid development of knowledge regarding how to manipulate and analyze DNA, including techniques for modifying its structure, has led to the new field in biology known as

A) graphic display.

B) DNA splicing.

C) recombinant DNA technology.

D) genetics.

E) electrophoresis.

Topic: Sec. 20.0

Bloom's: Remembering/Understanding

LO: 20.3a

GLO: G1

3) During DNA replication, a(n) ________ added to a DNA template serves as the beginning site of nucleotide addition.

A) initiation codon

B) 5' OH end of sugar group

C) 3' OH end of sugar group

D) primer

E) poly A tail

Topic: Sec. 20.1

Bloom's: Remembering/Understanding

LO: 20.1

GLO: G1

4) Each of the following is used in the process of DNA sequencing EXCEPT which one?

A) modified nucleotides

B) nucleotides

C) gel electrophoresis

D) RNA polymerase

E) DNA polymerase

Topic: Sec. 20.1

Bloom's: Remembering/Understanding

LO: 20.1

GLO: G1

5) After a strand of DNA has been cut with restriction enzymes, the pieces are attached again by the action of

A) DNA ligases.

B) stop codons or sequences.

C) DNA polymerase.

D) plasmids.

E) primers.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2a

GLO: G1

6) During polymerase chain reaction, DNA amplification can come to an end

A) when there are no more free nucleotides.

B) once the DNA template is used up.

C) once the RNA template is used up.

D) as DNA polymerase is produced.

E) when the stop codon is added to the template.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2b

GLO: G1

7) Bacterial ________ DNA can be easily removed from the bacterium, modified by adding a gene of interest, and then reinserted into the bacterium to be replicated.

A) genomic

B) polymerase

C) plasmid

D) chromosomal

E) nuclear

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2a

GLO: G1

8) A DNA sequence that can be read the same backward and forward is called a(n)

A) repetitive code.

B) palindrome.

C) plasmid sequence.

D) DNA fingerprint.

E) exon.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2a

GLO: G1

9) A scientist is working in a lab sequencing DNA. After reading the results from a column of gel scanned in a fluoroscope, she determines that the sequence of bases in the newly synthesized DNA is AATTCCCGG. Which one of the following represents the sequence of bases on the complementary strand?

A) GGCCAAATT

B) UUTTCCCGG

C) AATTCCCGG

D) UUAAGGGCC

E) TTAAGGGCC

Topic: Sec. 20.1

Bloom's: Applying/Analyzing

LO: 20.1

GLO: G2

10) Polymerase chain reaction (PCR) is useful for amplifying a small piece of DNA but is not a good technique for cloning genes because

A) DNA ligase cannot be used in PCRs.

B) PCRs do not generate palindromic sequences.

C) copies of DNA produced by PCRs lack the regulatory genes and proteins required to activate genes.

D) PCRs only generate primary transcripts that require excision of introns.

E) ribosomes are required to produce active genes.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2b

GLO: G1

11) What determines the specific segment of DNA that is amplified by PCR?

A) the plasmid used

B) the type of DNA polymerase

C) the use of DNA ligase

D) the source of nucleotides

E) the primers used

Topic: Sec. 20.2

Bloom's: Applying/Analyzing

LO: 20.2b

GLO: G2

12) Organisms that can contain genes introduced from other species are referred to as

A) bacteria.

B) plasmids.

C) manipulated.

D) genomic.

E) transgenic.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3a

GLO: G1

13) Which one of the following enzymes catalyzes the attachment of nucleotides to a growing complementary strand?

A) restriction enzyme

B) DNA polymerase

C) plasmid

D) DNA ligases

E) transgenic

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2b

GLO: G1

14) During PCR, heating of DNA is required to

A) promote attachment of nucleotides to the template.

B) allow DNA ligases to bind fragments together.

C) unwind double-stranded DNA.

D) attach the primer to the template DNA.

E) attach a stop codon.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.2b

GLO: G1

15) The following are steps involved in producing recombinant DNA using human DNA and a plasmid. Which one of the following answers best reflects the proper order of these steps?

1. select bacteria that contain human DNA

2. isolate and purify DNA to be recombined

3. add DNA ligase to join human DNA with plasmid

4. cut human DNA and plasmid with restriction enzyme

5. introduce new plasmid to bacteria

A) 4, 1, 5, 3, 2

B) 2, 4, 3, 5, 1

C) 2, 5, 1, 3, 4

D) 4, 3, 2, 1, 5

E) 5, 1, 2, 4, 3

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2a

GLO: G1

16) When DNA fragments from different sources are mixed together, they begin combining with each other due to

A) complementary base pairing.

B) the action of restriction enzymes.

C) a change in pH.

D) electrical currents present in the mixture.

E) a change in temperature.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2a

GLO: G1

17) A small amount of DNA was located at a crime scene. Which one of the following techniques is most likely to be used to amplify the DNA so it can be identified more accurately?

A) genetic engineering

B) use of DNA ligase to recombine the pieces of DNA

C) gene therapy

D) DNA sequencing

E) polymerase chain reaction

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2b

GLO: G1

18) Plants that have foreign DNA inserted into their genome for the purpose of producing products beneficial to humans are referred to as

A) recombinants.

B) mutated.

C) transgenic.

D) inferior.

E) genetically spliced.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

19) Prior to genetic engineering, insulin for humans with insulin-dependent diabetes was extracted from pigs and cattle. Presently insulin is produced by transgenic

A) humans.

B) chickens.

C) viruses.

D) bacteria.

E) monkeys.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

20) One technique used to yield a transgenic animal is to

A) amplify DNA from a donor animal and another organism of interest using PCR.

B) remove all of the DNA from an animal fetus, and insert new DNA of interest through the use of synthetic DNA "carriers."

C) microinject the DNA of interest into an animal's egg.

D) introduce proteins from a foreign organism into the blood of a pregnant animal, so that the protein sequences will be used to produce a new DNA template.

E) inject the DNA of interest into an animal's bone marrow.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3c

GLO: G1

21) Which one of the following human proteins, produced by transgenic bacteria, is used to prevent or break down blood clots?

A) insulin

B) tissue plasminogen activator

C) erythropoietin

D) growth hormone

E) factor VIII

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

22) Which one of the following explanations does not describe why generating transgenic animals is more difficult than plants?

A) The intracellular environment of an animal can be much different than bacteria and even plants.

B) Animal cells do not readily take up plasmid DNA like plant cells.

C) The techniques for cloning animals from a single cell are more challenging than for plants.

D) Genetic material must be inserted into a fertilized egg, then implanted into a living animal; plants can be grown in culture media, and thus, there is no need for implantation.

E) Genetic material is introduced to animal cells in culture media and the animal is allowed to mature in the culture media.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3c

GLO: G1

23) Which one of the following is an example of "gene pharming"?

A) the production of human insulin by E. coli

B) the production of beta carotene in golden rice

C) the production of goat milk that contains human antithrombin III protein

D) the production of HBV vaccines in yeast

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3c

GLO: G1

24) Which one of the following is a problem associated with traditional vaccines as opposed to vaccines produced by transgenic organisms?

A) Traditional vaccines are always in short supply.

B) Traditional vaccines may result in disease symptoms because they contain killed or weakened organisms.

C) Traditional vaccines can only be given after infection; they cannot prevent infection.

D) Traditional vaccines must be given in larger quantity.

E) Traditional vaccines are more expensive.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3a

GLO: G1

25) Insertion of a gene into another organism's DNA so that it can be transferred to another organism like a bacterium is called

A) vector transfer.

B) PCR.

C) DNA amplification.

D) gene therapy.

E) DNA translocation.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

26) Which one of the following genetically engineered vaccines is presently on the market?

A) vaccine for HIV

B) vaccine for malaria

C) vaccine for hepatitis B

D) vaccine for influenza

E) vaccine for human papillomavirus

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

27) Pharmaceutical companies prefer to genetically engineer large animals with human genes because it may be possible to obtain large amounts of the resulting protein from the animals'

A) milk.

B) fur.

C) blood.

D) meat.

E) bones.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3c

GLO: G1

28) One of the main problems associated with treating human diseases through gene therapy is

A) finding human cells that could potentially be genetically engineered.

B) the inability to clone human cells.

C) finding a delivery system that would target specific cell types.

D) finding the correct form of the gene to be delivered.

E) determining a disorder that could be cured through gene therapy.

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G1

29) The first successful treatment of a human genetic disorder through gene therapy was with a disorder known as

A) severe combined immunodeficiency disease.

B) diabetes mellitus.

C) human dwarfism.

D) hemophilia.

E) acquired immunodeficiency syndrome.

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G1

30) The first successful treatment of a genetic disorder by gene therapy was the curing of a 4-year-old girl who had severe combined immunodeficiency disease. Because the engineered cells did not live forever, this child supplemented her treatment with regular doses of

A) adenosine deaminase (ADA).

B) insulin.

C) hemoglobin.

D) bacteria.

E) B lymphocytes.

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G1

31) Gene therapy is currently under investigation as a possible way to cure cystic fibrosis. How is the viral vector carrying the normal gene delivered to the affected cells?

A) injection

B) bacterial plasmid

C) nasal spray

D) ingestion

E) application to skin

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G2

20.2 True/False Questions

1) The goal of polymerase chain reaction (PCR) is to determine the sequence of nucleotides in a stretch of DNA.

Topic: Sec. 20.1

Bloom's: Remembering/Understanding

LO: 20.1

GLO: G1

2) Through genetic engineering, it is possible for scientists to identify the sequence of bases in DNA, determine the location of certain genes in DNA, and create more DNA from DNA samples.

Topic: Sec. 20.0

Bloom's: Remembering/Understanding

LO: 20.1

GLO: G1

3) Plasmid DNA from humans has been successfully inserted and expressed in bacteria.

Topic: Sec. 20.1

Bloom's: Remembering/Understanding

LO: 20.1

GLO: G1

4) Small pieces of DNA can be amplified by a process known as polymerase chain reaction.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2b

GLO: G1

5) Gel electrophoresis is a technique used to separate a mixture of DNA fragments. During this process, large DNA fragments move longer distances than small DNA fragments when an electric current is applied.

Topic: Sec. 20.1

Bloom's: Remembering/Understanding

LO: 20.1

GLO: G1

6) The idea of manipulating DNA through cutting and splicing, essential to the science of recombinant DNA technology, is not necessarily a new process since nature has been recombining DNA for billions of years.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2a

GLO: G1

7) During PCR, the enzyme DNA ligase is added to the mixture to promote the addition of nucleotides to the growing DNA chain.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2b

GLO: G1

8) Restriction enzymes are used in genetic engineering to cut DNA between specific base pairs in a DNA strand, resulting in DNA segments that are then used for further study and analysis.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2c

GLO: G1

9) Plasmids are useful to scientists involved in recombinant DNA technology because DNA can be manipulated and inserted back into bacteria using them.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2a

GLO: G1

10) Understanding why normal cells commit suicide (apoptosis) may be the key to treating some forms of cancer.

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G1

11) Vaccines produced by genetically engineered bacteria cannot cause disease they are modeled after.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

12) Genetic modifications have been used to increase plant leaf size for better photosynthesis.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

13) Transgenic plants can presently be used for the production of human proteins, including human serum albumin.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

14) Gene therapy is a technique in which DNA from one species is inserted into a different species.

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G1

15) Producing transgenic animals is much easier than generating transgenic plants.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3c

GLO: G1

16) Once a genetic disorder in an adult is treated successfully through gene therapy, it is highly unlikely that the person's children will inherit the gene(s) for the disease.

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G1

20.3 Matching Questions

Recombinant DNA technology uses a number of "tools" and components. Match each of the following to its definition.

A) amplification of small pieces of DNA through a repeated series of cyclic biochemical reactions

B) a chemical typically isolated from bacteria that cuts DNA at specific sites, frequently producing palindromic ends

C) small, circular piece of DNA as vectors for transfer of DNA between organisms

D) a chemical that binds fragments of DNA together

E) a chemical facilitates the addition of nucleotides to a growing strand of DNA

1) polymerase chain reaction

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2b

GLO: G1

2) restriction enzyme

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2c

GLO: G1

3) DNA ligase

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2a

GLO: G1

4) plasmid

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2a

GLO: G1

5) DNA polymerase

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2b

GLO: G1

Answers: 1) A 2) B 3) D 4) C 5) E

20.4 Short Answer Questions

1) Technique of using plasmid DNA to insert another species DNA into a bacterium or plant is called ________ transfer.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

2) The science that involves the planned modification, insertion, or deletion of genes from the genetic makeup of an organism is called ________.

Topic: Sec. 20.0

Bloom's: Remembering/Understanding

LO: 20.3a

GLO: G1

3) To synthesize DNA in a laboratory, the enzyme called DNA ________ must be used to facilitate the addition of nucleotides on the new strand.

Topic: Sec. 20.1

Bloom's: Remembering/Understanding

LO: 20.1

GLO: G1

4) A(n) ________ is a sequence that reads the same from left to right on one strand of DNA as it does from right to left on the other strand and are common recognition sequences for restriction enzymes.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2c

GLO: G1

5) In recombinant DNA technology, DNA is cut at specific nucleotide sequences by ________ enzymes.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2c

GLO: G1

6) The technique used to make millions of identical copies of DNA from a small sample in a test tube is ________.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2b

GLO: G1

7) Small, circular DNA molecules that are located outside the main chromosome of bacteria and are capable of self-replication are ________.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2a

GLO: G1

8) ________ organisms are genetically modified or engineered to carry one or more foreign genes.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3a

GLO: G1

9) According to the text, the "workhorses" of the genetic engineering industry are ________.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

10) Transgenic bacteria are presently used to make ________ to protect from a possible infection by a disease-causing organism.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

11) Genetically engineered plants that contain high levels of beta-carotene are especially important additions to the diet because beta-carotene is converted to ________ in the human body.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3b

GLO: G1

12) Short single-stranded pieces of DNA called ________ attach to a DNA template to serve as the beginning site of DNA replication.

Topic: Sec. 20.1

Bloom's: Remembering/Understanding

LO: 20.1

GLO: G1

13) To create cows, sheep, and pigs that grow faster and larger, the gene for ________ has been inserted into their genomes.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3c

GLO: G1

14) The process of producing pharmaceuticals in farm animals is called gene ________.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3c

GLO: G1

15) The insertion of human genes into human cells to treat a disease is known as gene ________.

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G1

16) Gene therapy requires that genes be transports to cells and incorporated into the cells' DNA; ________ transfer genes into human cells.

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G1

17) Cystic fibrosis is a genetic disorder that is caused by the lack of a gene that encodes a cell membrane protein which transports ________ ions.

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G1

18) Programmed cell death in somatic cells that are damaged is known as ________.

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G1

19) Gene therapy may one day be used to cure some cancers. One approach currently under investigation is to add genes for ________ that stimulate the immune system (but not apoptosis). In this way, the immune system can target the cancer cells.

Topic: Sec. 20.4

Bloom's: Remembering/Understanding

LO: 20.4

GLO: G1

20) ________ is a useful technique in criminal investigations because humans contain many copies of repeating or junk sequences, the length of which can be unique to an individual.

Topic: Sec. 20.2

Bloom's: Remembering/Understanding

LO: 20.2c

GLO: G1

20.5 Essay Questions

1) Both plants and animals can be manipulated to generate transgenic organisms, but the process is much easier with plants. Explain why it is more difficult to generate transgenic animals than plants.

Topic: Sec. 20.3

Bloom's: Remembering/Understanding

LO: 20.3c

GLO: G8

2) The techniques associated with biotechnology have allowed for fairly widespread manipulation of food available for consumption. The idea of genetically modified foods has many Americans concerned about the safety of these foods. In your opinion, are the concerns well-founded?

Topic: Sec. 20.3, Current Issue

Bloom's: Applying/Analyzing

LO: 20.3b

GLO: G9

3) Gene therapy is showing some promise as a technique to help treat or prevent some diseases. Researchers have focused their attention on understanding more about the process of programmed cell death or apoptosis. Discuss why the process of apoptosis in injured or aging cells may be useful for understanding or treating cancer.

Topic: Sec. 20.4

Bloom's: Applying/Analyzing

LO: 20.4

GLO: G8