Genomics I: DNA Analysis – Ch22 Test Bank | 7e

View Product website:

https://selldocx.com/docx/genomics-i-dna-analysis-ch22-test-bank-7e-1311

Student name:__________

1) What would you expect to find in a metagenomic library? (Check all that apply.)

A) Peer-reviewed research articles on disease and the environment
B) The complete genome from a new species
C) Bits of DNA from numerous species
D) Many copies of a cloning vector with different inserts
E) A composite genome from several related species
F) Some DNA representing genes that are similar but differin exact sequence

2) Which researcher(s) are using a metagenomic approach? (Check all that apply.)

A) A medical researcher studies the DNA of mixed bacterial populations from the intestines of patients with different diseases.
B) A plant biologist sequences the genomes of broccoli, cauliflower, and other Brassica species to determine which genes are responsible for their different features.
C) A developmental geneticist clones the mutated gene from a Drosophila strain with disrupted wing development.
D) An environmental researcher sequences DNA from collections of microbes living in polluted coastal waters, which cannot be cultured in the lab.
E) A pharmaceutical developer screens through DNA found in soil samples for novel antibiotics.

3) A woman has a child and wants to determine who the father of the child is. There are four possible fathers. This group of individuals is analyzed with regard to three different STSs: STS-1 is 215 bp and 199 bp, STS-2 is 144 bp and 122 bp, and STS-3 is 101 bp and 85 bp. The child (C), mother (M), and 4 potential dads (D1-D4) show the following results. Select the individual(s) who are heterozygous for STS-3. (Check all that apply.)

A) C
B) M
C) D1
D) D2
E) D3
F) D4

4) Which of the following procedures best determines the relative order, but not precise location, of a series of genes on a chromosome?

A) Physical mapping
B) Cytogenetic mapping
C) Linkage mapping

5) ________ uses the unique banding patterns of a chromosome to determine the general location of a gene.

A) Physical mapping
B) Cytogenetic mapping
C) Linkage mapping

6) What does physical mapping provide that the other types of genetic mapping do not?

A) Recombination distances between genes
B) Number of nucleotide base-pairs between genes
C) Relationship of the gene to specific chromosomal banding patterns

7) The process of in situ hybridization is used for which of the following?

A) Cytogenetic mapping
B) Physical mapping
C) Linkage mapping

8) Which of the following acronyms describes a technique used in chromosome painting?

A) RFLP
B) VNTR
C) STR
D) FISH
E) STS

9) A site that has variation within the members of the population is said to be __________.

A) monomorphic
B) polymorphic
C) trimorphic

10) The most common microsatellite in humans is __________.

A) (AT) 100
B) (CA) n
C) (TG) n
D) (CAT) n

11) The method of sequencing genomic fragments that are randomly generated from larger DNA fragments is called __________.

A) cytogenetic mapping
B) shotgun sequencing
C) Southern blotting
D) polymerase chain reaction

12) The human genome project's first draft took approximately _______ years.

A) 5
B) 13
C) 20
D) 50
E) 2

13) The human genome consists of approximately _______ base pairs of DNA.

A) 100,000
B) 1 million
C) 1 billion
D) 3 billion
E) 2 trillion

14) A series of clones that contain overlapping pieces of a chromosome are called a _______.

A) contig
B) artificialchromosome
C) cosmid
D) positionalclone
E) transposableelement

15) A _______ is a combination of a plasmid vector and phage λ.

A) contig
B) artificialchromosome
C) cosmid
D) positionalclone
E) transposableelement

16) In shotgun sequencing, how is a complete sequence of a genome reconstructed?

A) The entire genome is directly sequenced chromosome by chromosome.
B) The genome is fragmented and sequences of each fragment are arranged so that overlapping regions are identified.
C) The sequences of a large number of individuals is obtained and “averaged” to obtain the complete sequence.
D) The position of each marker and gene are first mapped relative to regions for which the genomic sequence is not known.

17) Which of the following is the most direct benefit of high-throughput Sanger sequencing?

A) Bases can be identified as soon as they are added to the template.
B) RNA can be directly sequenced without first reverse transcribing it into DNA.
C) An entire chromosome can be sequenced without disrupting it into smaller fragments.
D) Many DNA samples can be sequenced simultaneously.

18) Which of the following samples would NOT be suitable for a metagenomics-based approach?

A) Water samples from different bodies of water with different temperatures, salinities, and acidities
B) A soil sample from a farm where a new planting schedule has been implemented
C) A water sample from an industrial site that has been contaminated with pollutants
D) A fecal sample from a patient with irritable bowel syndrome
E) A homogeneous population of skin cancer cells collected from a patient biopsy

19) In positional cloning, which method is used to identify clones that are progressively closer to a gene of interest?

A) Chromosome painting
B) Cytogenetic mapping
C) Microsatellite analysis
D) Chromosome walking

20) The experimental process of determining the relative location of genes or other segments of DNA along individual chromosomes is called __________.

A) mapping
B) metagenomics
C) DNA sequencing
D) proteomics

21) You are sequencing the genome of the yeast S. cerevisiae, with a genome size of 12,100,100 bp, using shotgun sequencing. Your sequencer malfunctions when only 24,200,000 bp in total have been sequenced. How much of the genome should have been sequenced at this point?

A) 100%
B) 0%
C) 13.5%
D) 86.5%
E) 0.135%
F) 0.865%

22) A woman has a child and wants to determine who the father of the child is. There are four possible fathers. This group of individuals is analyzed with regard to three different STSs: STS-1 is 215 bp and 199 bp, STS-2 is 144 bp and 122 bp, and STS-3 is 101 bp and 85 bp. The child (C), mother (M), and 4 potential dads (D1-D4) show the following results. Which individual is the father of the child?

A) D1
B) D2
C) D3
D) D4

23) You are carrying out a chromosome painting experiment. You have single-stranded DNAs that bind to 6 different regions of the X chromsomsome. You inadvertantly label the DNA probes all with the same colored fluorescent molecule, which happens to be red in this experiment. What will be the result of your experiment?

A) The DNA probes will be unable to bind.
B) The probes will bind, but you will not be able to tell which spot corresponds to which sequence.
C) The entire X chromosome will be labeled red along its entire length.
D) The probes will bind normally, and you will still be able to distinguish between the probes.

24) Imagine that when shotgun sequencing was intially used to sequence the H. influenzae genome, the sonicator didn't work. How would this affect the sequencing experiment?

A) Because the DNA fragments would be longer than what is usually carried by a plasmid DNA, no sequence would be obtained.
B) Because the DNA fragments would be longer than what is usually carried by a plasmid DNA, there would likely be sequences missing in the sequencing results due to difficulties with DNA replication.
C) Because the DNA fragments would be longer than what is usually carried by a plasmid DNA, sequencing would occur more quickly than usual.
D) Because the DNA fragments would be shorter than what is usually carried by a plasmid DNA, there would likely be sequences missing in the sequencing results due to difficulties with DNA replication.
E) Because the DNA fragments would be shorter than what is usually carried by a plasmid DNA, no sequence would be obtained.
F) Because the DNA fragments would be shorter than what is usually carried by a plasmid DNA, sequencing would occur more quickly than usual.

25) Map units correspond to fixed physical distances.

⊚ true
⊚ false

26) An RFLP marker may be used to predict the likelihood that an individual carries a trait for a specific disease.

⊚ true
⊚ false

27) Metagenomics refers to the molecular analysis of the entire genome of a species.

⊚ true
⊚ false

28) BAC stands for bacterial artificial chromosome.

⊚ true
⊚ false

29) In a FISH procedure, the probe being used is labeled with a fluorescent tag.

⊚ true
⊚ false

30) Cytogenetic mapping is accurate to within 500,000 base pairs, but not over this value.

⊚ true
⊚ false

31) Comparative genomics attempts to define evolutionary relationships.

⊚ true
⊚ false

32) Shotgun sequencing is precise enough to achieve a complete genomic sequence without the need for other mapping methods.

⊚ true
⊚ false

33) In sequencing by synthesis (SBS) methods, the sequence of a sample is directly determined as nucleotides are incorporated into a DNA strand by DNA polymerase.

⊚ true
⊚ false

34) Metagenomics is a common approach for studying the genes of a clonal population of bacteria in the laboratory.

⊚ true
⊚ false

35) YAC stands for yeast artificial cosmid.

⊚ true
⊚ false

36) To map a gene by chromosome walking, the sequence of the gene to be mapped is required.

⊚ true
⊚ false