Test Questions & Answers Gene Expression And Epigenetics Ch2

Chapter 2: Gene Expression and Epigenetics

Multiple Choice

1. DNA sequences that are involved in the regulation of gene expression are called

A. cis factors.

B. trans factors.

C. attenuators.

D. repressors.

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2. Which of the following is the binding site for the repressor protein of the lactose operon?

A. P site

B. Operator

C. A site

D. Promoter

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3. Which of the following would prevent transcription of the lactose operon?

A. Loss of promoter

B. Presence of inducer

C. Loss of the repressor protein

D. RNA polymerase binding to the promoter

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4. Which of the following is a cis factor of the lactose operon?

A. Inducer

B. Operator

C. Repressor

D. Polymerase

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5. In the lactose operon, which of the following configurations would result in gene expression?

A. Promoter +, Operator +, Repressor +, no inducer present

B. Promoter –, Operator +, Repressor +, no inducer present

C. Promoter –, Operator –, Repressor +, inducer present

D. Promoter +, Operator +, Repressor –, no inducer present

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6. When gene expression is regulated by mechanisms other than the interaction of cis elements and trans factors, the regulation is called

A. induction.

B. epigenetics.

C. attenuation.

D. combinatorial control.

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7. Genomic imprinting is accomplished primarily through

A. methylation.

B. acetylation.

C. transcription.

D. cis and trans factor interactions.

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8. The most frequently methylated base in vertebrates is

A. adenine.

B. cytosine.

C. guanine.

D. thymine.

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9. MicroRNAs, short endogenous RNAs, perform what function in the eukaryotic cell?

A. Control of DNA replication

B. Priming RNA synthesis

C. RNA splicing

D. Repression of gene expression

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10. What mechanism may explain the difference in symptoms in Prader–Willi and Angelman syndromes?

A. Genomic imprinting

B. Alternative splicing

C. Genetic recombination

D. Capping

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11. Alteration of the nucleotide sequence of RNA after transcription is called

A. methylation.

B. RNA silencing.

C. RNA editing.

D. capping.

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12. Which RNA results from exogenous nucleic acids?

A. siRNA

B. lncRNA

C. mRNA

D. miRNA

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13. Which DNA methyltransferase (DNMT) is responsible for maintenance of DNA methylation patterns?

A. DNMT3A

B. DNMT2

C. DNMT1

D. DNMT3B

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14. Which of the following is an epigenetic mechanism?

A. Binding of transcription factors

B. DNA hairpin formation

C. Cis factors

D. DNA methylation

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15. Which is a histone mark?

A. Binding of histone H1 to euchromatin

B. A modified nucleosime

C. Histone H3 tail sequence

D. Acetylation of histone H3

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16. Which histone is not included in the core histones?

A. H1

B. H2B

C. H3

D. H4

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17. This is a list of histone modifications that affect gene expression.

A. Transcriptome

B. Histone code

C. Proteome

D. Genetic code

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18. Methylation of lysine at position 20 in histone 2B is expressed in this way.

A. K20H2BMe

B. H2BK20Me

C. MeK20H2B

D. H2K20Me

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19. Acetylation of histone H3 would result in which of the following effects?

A. Degradation of mRNA

B. Formation of heterochromatin

C. Exclusion of activator proteins

D. Increased gene expression

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20. These DNA sequence structures function in epigenetic gene regulation.

A. Operators

B. CpG islands

C. Inverted repeats

D. Regulator genes

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21. Which proteins perform demethylation of DNA?

A. DNA polymerases

B. TET enzymes

C. METTL enzymes

D. TFIID protein

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22. This complex is required for miRNA and siRNA association to mRNA targets.

A. Replisome

B. Retrotransposon

C. RISC

D. XIC

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23. Enhancer regulation of gene expression is facilitated by this RNA.

A. lncRNA

B. miRNA

C. siRNA

D. Ri Xist RNA

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24. DNA hypermethylation would have what effect of movement of retrotransposons through RNA intermediates?

A. Increase movement by activating transcription

B. Increase movement by repressing transcription

C. No effect on movement nor transcription

D. Inhibiy movement by repressing transcription

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25. Multiple products are generated from the same gene by what mechanism?

A. Alternative splicing

B. Polyadenylation

C. Capping

D. Splicing